Journal: Molecules

Article Title: Ulva intestinalis Protein Extracts Promote In Vitro Collagen and Hyaluronic Acid Production by Human Dermal Fibroblasts

doi: 10.3390/molecules25092091

Figure Lengend Snippet: Figure 5. Effect of PROT-1 and H-PROT-1 on fibroblast proliferation in vitro (MTT assay). Results are expressed as the mean relative percentage of viable fibroblasts compared to the negative control (100%). Significant differences between values obtained with samples and negative control (n = 20) are indicated by * (p < 0.05), ** (p < 0.01) and *** (p < 0.001).

Article Snippet: Human dermal fibroblasts (CCD-1059Sk, lot number 62062292, and CCD-1090Sk, lot number 204756) were obtained from ATCC Cell Figure 9. yaluronic acid production per ell and per cell by the hu an der al fibroblast cell line 1059.

Techniques: In Vitro, MTT Assay, Negative Control

Journal: Molecules

Article Title: Ulva intestinalis Protein Extracts Promote In Vitro Collagen and Hyaluronic Acid Production by Human Dermal Fibroblasts

doi: 10.3390/molecules25092091

Figure Lengend Snippet: Figure 6 shows the effect of the PROT-1 fraction and H-PROT-1 hydrolysates on the cell mortality by necrosis, compared to the negative control. PROT-1 had no effect on cell mortality while a slight decrease in cell mortality was observed with H-PROT-1 at t2h, t4h and t6h: −8% (cell line 1059) and −7% (cell line 1090) for example at t4h. Except for the highest concentration of PROT-1, 500 µg/mL, which led to a very slight increase in cell mortality (+7% for the 1059-cell line and +9.5% for the 1090-cell line), no deleterious effect was observed on fibroblasts. Overall, PROT-1 decreased cell proliferation without increasing cell mortality by necrosis. Besides, we did not observe any alteration of the fibroblast morphology when checking the cells with optical microscopy. PROT-1 thus appeared to have a cytostatic effect on both cell lines in a similar way, but this effect was reduced after enzymatic protein hydrolysis. This would therefore indicate that the molecular weight or the distinctive structure of some PROT-1 proteins is related to this cytostatic effect.

Article Snippet: Human dermal fibroblasts (CCD-1059Sk, lot number 62062292, and CCD-1090Sk, lot number 204756) were obtained from ATCC Cell Figure 9. yaluronic acid production per ell and per cell by the hu an der al fibroblast cell line 1059.

Techniques: Negative Control, Concentration Assay, Microscopy, Molecular Weight

Journal: Journal of oleo science

Article Title: The anti-proliferative effects of a palm oil-derived product and its mode of actions in human malignant melanoma MeWo cells.

doi: 10.5650/jos.61.227

Figure Lengend Snippet: Fig. 3 Anti-proliferative effect of Bio-12 on cell growth. Dose–response curves of the anti-proliferative activity of Bio-12 (Control, 1/500, 1/200, 1/100, 1/10 and neat) on (A) MeWo, (B) HaCaT and (C) CCD-1059sk cells after 24, 48 and 72 h of treatments. Results are expressed as the percent- age of cell viability relative to the control. Each value is the mean ± S.D of three determinations. Significant inhibition of cell proliferation of Bio-12, towards MeWo cells was at of 1/100, 1/50, 1/10 and neat for 24 h; and 1/500, 1/200, 1/100, 1/50 and 1/10 for 48 and 72 h with an IC50 value 1/25 after 72 h. For the HaCaT cells, the cell proliferation was signifi cantly inhibited at 1/100, 1/150, 1/10 and neat for 24 and 48 h; and 1/200, 1/100, 1/50, 1/10 and neart for 72 h. The asterisk indicates a signifi cant difference be- tween control and Bio-12 treated cells, *p<0.05.

Article Snippet: The human malignant melanoma cell line(MeWo)(ATCC# HTB-65)and human normal skin fi broblasts(CCD-1059sk) (ATCC# CRL-2072)were purchased from the American Type Tissue Culture Collection(ATCC), Manassas, USA.

Techniques: Activity Assay, Control, Inhibition